
Understand the Science Behind Biofilm Detection with Carbotect™



Understand the Science Behind Biofilm Detection with Carbotect™



Innovative Solutions
What is Carbotect™?
Carbotect™ is a colour-based test designed to detect residual organic compounds and associated biofilms following incomplete cleaning. The Carbotect™ test validates the CIP cleaning of closed systems, and uses a substantially more representative water sample (100ml) for testing when contrasted against ATP based swab tests. The test is simple. Collect a 100ml sample of the final rinse water after cleaning, add the Carbotect™ reagent powder to the sample bottle as supplied and wait 5 minutes for the resulting colour reaction. The colour change is compared against the proprietary colour chart to confirm the sanitary status of the rinse water and to semi-quantify the presence of organic compounds after incomplete cleaning and/or biofilm presence.
Biofilm detection
Biofilms become established when environmental conditions are suitable to support microbial growth. A key to biofilm growth is the supply of nutrients associated with incomplete cleaning of process equipment. Biofilm detection and monitoring is an ongoing challenge and CarbotectTM readily detects the organic components from biofilms present in production and packaging systems.



Key Advantages of Carbotect™
Immediate Results
Experience immediate validation of cleanliness, ensuring swift hygiene compliance in high-risk operations
User-Friendly Kits
Our composite testing kits are designed for simplicity, allowing for quick training and testing for consistently reliable results.
High Accuracy
Carbotect™ delivers repeatable outcomes allowing you to rapidly identify and semi-quantify sources of organic contaminants in your process.
Regulatory Compliance
Aligns with and complements industry standards, supporting the development of robust hygiene protocols to address evolving challenges.

Key Advantages of Carbotect™
Immediate Results
Experience immediate validation of cleanliness, ensuring swift hygiene compliance in high-risk operations
User-Friendly Kits
Our composite testing kits are designed for simplicity, allowing for quick training and testing for consistently reliable results.
High Accuracy
Carbotect™ delivers repeatable outcomes allowing you to rapidly identify and semi-quantify sources of organic contaminants in your process.
Regulatory Compliance
Aligns with and complements industry standards, supporting the development of robust hygiene protocols to address evolving challenges.


Connect with Us for Immediate Insights
Learn how Carbotect™ can enhance your operations.
Reach out today for personalized guidance and support.

Connect with Us for Immediate Insights
Learn how Carbotect™ can enhance your operations.
Reach out today for personalized guidance and support.
The Carbotect™ technology has been:
Developed and patented for the rapid detection of residual organic contaminants and biofilms in general processing equipment.
Refined to detect ultra-low concentrations of microbial Exopolysaccharides (EPS) associated with biofilms present in process, product and domestic water systems.
Established as a complementary measure of evolving hygiene and sanitary standards in accordance with the demands of best practice, optimal safety and sustainability objectives.
Integrated as a sentinel test of the sanitary effectiveness of a CIP cleaning procedure as well as a semi-quantitative measure of the degree of organic contamination.
Developed as a proactive detection tool to identify biofilm presence, and to monitor the performance of cleaning and sanitation.
Designed as a dedicated and sentinel safeguard for product integrity, reputation and brand equity.
Used to identify and isolate sources of contamination and identify aspects of non-hygienic engineering design in the plant infrastructure.
Adapted as an ancillary diagnostic tool for high-risk processing facilities to detect potential food allergens.
The Carbotect™ technology has been:
Developed and patented for the rapid detection of residual organic contaminants and biofilms in general processing equipment.
Used to identify and isolate sources of contamination and identify aspects of non-hygienic engineering design in the plant infrastructure.
Developed as a proactive detection tool to identify biofilm presence, and to monitor the performance of cleaning and sanitation.
Established as a complementary measure of evolving hygiene and sanitary standards in accordance with the demands of best practice, optimal safety and sustainability objectives.
Integrated as a sentinel test of the sanitary effectiveness of a CIP cleaning procedure as well as a semi-quantitative measure of the degree of organic contamination.
Refined to detect ultra-low concentrations of microbial Exopolysaccharides (EPS) associated with biofilms present in process, product and domestic water systems.
Adapted as an ancillary diagnostic tool for high-risk processing facilities to detect potential food allergens.
Designed as a dedicated and sentinel safeguard for product integrity, reputation and brand equity.


Frequently Asked Questions
Common Queries About Carbotect™ Results and Usage
Carbotect is used to validate equipment cleaning and to detect organic residues associated with biofilm growth and product spoilage.
Carbotect reacts with the free organic components of the biofilm matrix which protects and supports unseen microbial growth.
The Carbotect test uses a substantially more representative sample volume (1000x more) to detect the presence of organic molecules. These residues may not include ATP molecules and thus excludes false negative results from cleaning validation tests.
Any organic food allergen residues in post-cleaning rinse water will be detected and confirms an adverse hygiene outcome with associated consumer risks.
Any person with hygiene responsibility and appropriate training within a production facility can perform the test. This conforms with other on-site tests routinely conducted by factory personnel.
The test result with significant cleaning failure is immediate. The final cleaning validation and semi-quantification of residue persistence requires 5 minutes of exposure to the reagent before comparison with the proprietary colour chart.
A 100ml sample of the final rinse water from the ‘cleaned’ equipment is collected for the Carbotect™ test.
Conventional microbiological validation of hygiene is time dependent with a constraining lag period before definitive outcomes become available. Alternatively rapid testing associated with low volume sampling of water or surface areas lacks representativity of all contact surfaces and limits integrity of hygiene validation.
Any and all industries that rely on clean water to perform a final equipment rinse before production can embrace Carbotect™ for validation of cleaning and sanitation efficiency.
The test is only performed on a sample of final rinse water and has no product contact. This testing procedure does not need regulatory approval or certification.
No special laboratory equipment or facilities are required to conduct the test.
Frequently Asked Questions
Common Queries About Carbotect™ Results and Usage
Carbotect is used to validate equipment cleaning and to detect organic residues associated with biofilm growth and product spoilage.
Carbotect reacts with the free organic components of the biofilm matrix which protects and supports unseen microbial growth.
The Carbotect test uses a substantially more representative sample volume (1000x more) to detect the presence of organic molecules. These residues may not include ATP molecules and thus excludes false negative results from cleaning validation tests.
Any organic food allergen residues in post-cleaning rinse water will be detected and confirms an adverse hygiene outcome with associated consumer risks.
Any person with hygiene responsibility and appropriate training within a production facility can perform the test. This conforms with other on-site tests routinely conducted by factory personnel.
The test result with significant cleaning failure is immediate. The final cleaning validation and semi-quantification of residue persistence requires 5 minutes of exposure to the reagent before comparison with the proprietary colour chart.
Conventional microbiological validation of hygiene is time dependent with a constraining lag period before definitive outcomes become available. Alternatively rapid testing associated with low volume sampling of water or surface areas lacks representativity of all contact surfaces and limits integrity of hygiene validation.
Any and all industries that rely on clean water to perform a final equipment rinse before production can embrace Carbotect™ for validation of cleaning and sanitation efficiency.
The test is only performed on a sample of final rinse water and has no product contact. This testing procedure does not need regulatory approval or certification.
No special laboratory equipment or facilities are required to conduct the test.
Intellectual property
The proprietary Carbotect™ technology is currently protected by the following patents:
Get in Touch
We are here to help you with any questions you may have about our services or technology. Your inquiries matter to us. Reach us with your queries anytime by emailing info@carbotect.com or filling the form below: